Chromatography and Mass Spectrometry Reagents

Use Thermo Scientific™ MSTFA and MSTFA + 1% TMCS Silylation Reagent for GC analyses of early eluting compounds that would otherwise be obscured in the chromatogram. MSTFA, the most volatile TMS-amide available, and its byproduct N-methyltrifluoroacetamide typically elute with the solvent front,...

To siliconize glass surfaces use Thermo Scientific™ Water-Soluble Siliconizing Fluid. This easy-to-use silane monomer solution is supplied as a 20% solid solution in a mixture of diacetone alcohol and tertiary butyl alcohol.

Derivatize hydroxyls compounds, particularly carbohydrates with Thermo Scientific™ Tri-Sil TP (TMSI:Pyridine) Reagent. Comprising TMSI in pyridine, this reagent silylates alcohols and phenols, organic acids, hydroxylamines, amino acids, carbohydrates, flavonoids, glycols and polyglycols,...

Employ a reagent-catalyst solvent mixture, for one-step derivatization with the Thermo Scientific™ Tri-Sil HTP (HDMS:TMCS:Pyridine) Reagent, Composed of HMDS, TMCS and high-purity pyridine, it rapidly produces TMS derivatives of polar compounds for GC analysis and biochemical synthesis.

Produce stable TBDMS derivatives for GC analysis with the Thermo Scientific™ MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent. MTBSTFA derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines with typically >96% yields, and the reaction byproducts are volatile and neutral.

Prepare oximes of steroids and ketoacids prior to silylation with the Thermo Scientific™ Methoxamine (MOX) Reagent. It prevents the formation of multiple derivatives when enols are present during silylation, thereby ensuring optimal quantitation.

Use Thermo Scientific™ BSTFA + 1–10% TMCS Silylation Reagent for analytes that are difficult to silylate. Stronger than BSTFA alone, this catalyzed formulation is ideal for derivatizing compounds such as fatty acid amides and slightly hindered hydroxyls for GC analysis.

Prepare oximes of steroids and ketoacids prior to silylation with the Thermo Scientific™ Methoxamine (MOX) Reagent. It prevents the formation of multiple derivatives when enols are present during silylation, thereby ensuring optimal quantitation.

For accurate, sensitive, on-column methylation and detection by GC analysis use Thermo Scientific™ MethElute™ Reagent (TMPAH). It is ideally suited for the quantitative methylation and detection of barbiturates, sedatives, xanthine bases, phenolic alkaloids, and phenytoin, giving a single...

Employ a catalyzed silylation reagent formulation, for derivatization of hydroxyls with the Thermo Scientific™ Tri-Sil TBT (TMSI:BSA:TMCS) Reagent. Composed of TMSI/BSA/TMCS (3:3:2), this catalyzed silylation reagent formulation typically reacts with hydroxyls in a short period of time at 60–80 °C...

Improve GC analysis with Thermo Scientific™ BSTFA Silylation Reagent. The highly volatile byproducts mono(trimethylsilyl)trifluoroacetamide (BSTFA) and trifluoroacetamide elute with the solvent front to ensure excellent chromatographic separations.

Use specially manufactured and packaged Thermo Scientific™ Silylation Grade Solvents to meet the exacting needs of silylation and other sensitive derivatization reactions. They are purified, dried, and packaged under nitrogen in convenient Thermo Scientific™ Hypo-Vial™ Sample Storage Vials, and are...

Employ a catalyzed silylation reagent formulation, for derivatization of hydroxyls with the Thermo Scientific™ Tri-Sil TBT (TMSI:BSA:TMCS) Reagent. Composed of TMSI/BSA/TMCS (3:3:2), this catalyzed silylation reagent formulation typically reacts with hydroxyls in a short period of time at 60–80 °C...

To siliconize glass surfaces use Thermo Scientific™ Water-Soluble Siliconizing Fluid. This easy-to-use silane monomer solution is supplied as a 20% solid solution in a mixture of diacetone alcohol and tertiary butyl alcohol.