Buffers and Standards

Iodine, 0.1N standardized solution is used for accurate volumetric analysis. It is also used as a disinfectant and antibacterial agent in solutions, tinctures and salves. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio.

Citrate, is used as an anticoagulant and also used as a biological buffer. It is often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus, unmasking antigens and epitopes in formalin-fixed and paraffin...

Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio.

6.0N HClsolution acts as a standard solution in volumetric titrations. In addition, it has industrial applications such as chemical transformations in hydrochlorination of rubber and production of vinyl and alkyl halide.

MOPS is mainly used in biological experiments as a buffer solution. It is also used in protein purification. Further, it is utilized for polyacrylamide gel electrophoresis. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio.

Commonly used buffering agent

PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4

Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.)

DNase-, RNase- and Protease-Free.

1 mL = 62.4 mg CuSO4.5H2O

Tris-EDTA (TE) is routinely used for suspending nucleic acid samples.

Commonly used buffering agent