Acrylamide, electrophoresis grade, 99+%

Acrylamide / N,N'-Methylenebisacrylamide, CAS # 79-06-1, 7732-18-5, 110-26-9, 79-06-1, is a water soluble compound that cross-links to produce polyacrylamide gels that are widely used in chromatography and the electrophoretic separation of nucleic acids and proteins.

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500 g

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Catalog number ALFL15075.36

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500 g

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Chemical Identifiers

CAS118-75-2

Specifications

Specification Sheet

Appearance (Color)Yellow to yellow-green

Appearance (Form)Powder or crystals

Infrared spectrumConforms

HPLC>=98.5 %

Loss on drying=<0.5 % (1 g, 105°C, 3 hrs)

Acrylamide is used as a monomer in the synthesis of polyacrylamides, which finds application as water-soluble thickeners in waste water treatment, gel electrophoresis, ore processing and dyes production. In molecular biology, it is used as a carrier as well as involved in the precipitation of small amounts of DNA. It plays an important role as an intermediate in the preparation of N-methylol acrylamide and N-butoxyacrylamide.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

Acrylamide / N,N'-Methylenebisacrylamide is a water-soluble, odorless, white crystalline powder
This compound polymerizes in the presence of free radicals to generate gels whose pore-size may be regulated by manipulating the relative concentrations of this mixture of compounds

Applications

Polyacrylamide gels are widely used in biochemistry, forensic chemistry, genetics, molecular biology, and biotechnology to separate proteins or nucleic acids, according to their electrophoretic mobility
Because of their higher resolution compared to other gels, polyacrylamide gels are also used in the analysis of smaller RNA species from mixed samples

RUO – Research Use Only

General References:

Petrov, A.; Tsa, A.; Puglisi, J.D. Chapter Sixteen – Analysis of RNA by Analytical Polyacrylamide Gel Electrophoresis. Methods in Enzymology. 2013, 530, 301–313.
Shapiro, A. L.; Viñuela, E.; Maizel, J. V. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem. Biophys. Res. Commun. 1967, 28 (5), 815–20.
Duchesne, L. G.; Lam, J. S.; MacDonald, L. A.; et al. Effect of pH and acrylamide concentration on the separation of lipopolysaccharides in polyacrylamide gels. Current Microbiology. 1988, 16 (4), 191–4.