5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%, Thermo Scientific Chemicals
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%, Thermo Scientific Chemicals
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%, Thermo Scientific Chemicals
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%, Thermo Scientific Chemicals
Thermo Scientific Chemicals

5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%, Thermo Scientific Chemicals

Substrate for alkaline phosphatase
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Catalog NumberQuantity
ALFJ64451.031 g
Catalog number ALFJ64451.03
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Quantity:
1 g
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Chemical Identifiers
CAS844891-04-9
SpecificationsSpecification SheetSpecification Sheet
Assay from Supplier's CofA≥94.0%
Appearance (Color)White
FormCrystals or powder or crystalline powder
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, is used for the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, is used for the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

Solubility
Soluble in water (50 mg/mL). Insoluble in dimethylformamide.

Notes
Moisture & Light Sensitive. Keep container tightly closed. Store in cool, dry conditions in well sealed containers.
RUO – Research Use Only

General References:

  1. Chuanmin Ruan.; Kefeng Zeng.; Oomman K Varghese.; Craig A Grimes. A magnetoelastic bioaffinity-based sensor for avidin. Biosensors and Bioelectronics. 2004, 19, (12), 1695-1701.
  2. Chuanmin Ruan.; Liju Yang.; Yanbin Li. Immunobiosensor Chips for Detection of Escherichia coli O157:H7 Using Electrochemical Impedance Spectroscopy. Anal. Chem. 2002, 74, (18), 4814-4820.