TAE (50X), TRIS + acetate + EDTA
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TAE (50X), TRIS + acetate + EDTA
Thermo Scientific Chemicals

TAE (50X), TRIS + acetate + EDTA

TAE (50X), TRIS + acetate + EDTA is a buffer composed of Tris, acetate, and EDTA components and is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.
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Catalog number ALFJ63931.K3
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Chemical Identifiers
CAS2390-54-7
IUPAC Name2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium chloride
Molecular FormulaC17H19ClN2S
InChI KeyJADVWWSKYZXRGX-UHFFFAOYSA-M
SMILES[Cl-].CN(C)C1=CC=C(C=C1)C1=[N+](C)C2=CC=C(C)C=C2S1
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SpecificationsSpecification SheetSpecification Sheet
Infrared spectrumConforms
Appearance (Color)Yellow to golden to orange-brown
UV Lambda max409 to 415 nm (10 mg/l in water)
Appearance (Form)Powder
Solubility(1 mg/ml in methanol) Clear to slightly hazy yellow to yellow-green
TAE (50X), TRIS + acetate + EDTA is used in electrophoresis of nucleic acids in agarose and polyacrylamide gel. It acts as a running buffer and as a gel preparation buffer. It is also useful for nucleic acid separations viz. DNA and RNA. Further, it is used for non-denaturing RNA agarose gel electrophoresis.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• TAE (50X), TRIS + acetate + EDTA allows nucleic acid separation

Application

• This buffer has been used to prepare agarose and polyacrylamide gels for electrophoresis of nucleic acids
• It is used as a running and gel preparation buffer, and it is also used for non-denaturing RNA agarose gel electrophoresis

RUO – Research Use Only

General References:

  1. Lechanteur, A.; Furst, T.; Evrard, B.; Delvenne, P.; Hubert, P.; Piel, G. Development of anti-E6 pegylated lipoplexes for mucosal application in the context of cervical preneoplastic lesions. Int. J. Pharm. 2015, 483 (1-2), 268-277.
  2. Kaur, K.; Kumar, V.; Beniwal, V.; Kumar, V.; Aneja, K. R.; Sharma, V.; Jaglan, S. T. Solvent-free synthesis of novel (E)-2-(3, 5-dimethyl-4-(aryldiazenyl)-1H-pyrazol-1-yl)-4-arylthiazoles: determination of their biological activity. Med. Chem. Res. 2015, 24 (11), 3863-3875.