o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals
o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals
o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals
o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals
Thermo Scientific Chemicals

o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals

A peroxidase substrate for ELISA procedures
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Catalog NumberQuantity
ALFJ60354.1425 g
Catalog number ALFJ60354.14
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Quantity:
25 g
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Specifications
Chemical Name or Materialo-Phenylenediamine dihydrochloride
CAS615-28-1
Health Hazard 1H301-H312+H332-H317-H319-H341-H351
Health Hazard 2GHS H Statement
H301-H341-H351-H312-H332-H319-H317
Toxic if swallowed.
Suspected of causing genetic defects.
Suspected of causing cancer.
Harmful in contact with skin.
Harmful if inhaled.
Causes serious eye irritation.
May cause an allergic skin reaction.
Health Hazard 3P201-P202-P261-P264b-P270-P271-P272-P280-P281-P301+P310-P302+P352-P304+P340-P305+P351+P338-P308+P313-P312-P330-P333+P313-P363-P501c
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o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Solubility
Slightly soluble in water; freely soluble in alcohol, chloroform or ether

Notes
Store in cool, dry place in a tightly sealed container. Store away from oxidizing agents, heat. Store in refrigerator.
RUO – Research Use Only

General References:

  1. J H Bovaird; T T Ngo; H M Lenhoff. Optimizing the o-phenylenediamine assay for horseradish peroxidase: effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents. Clinical Chemistry.1982, 28 (12), 2423-2426.
  2. G Wolters; L Kuijpers; J Kacaki; A Schuurs.Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. Journal of Clinical Pathology. 1976, 29 (10), 873-879.