OPTIZYME™ DNase I (RNase-Free), Fisher BioReagents™

Name: OPTIZYME™ DNase I (RNase-Free), Fisher BioReagents™
SKU: FGCBP8107-1

Catalog number FGCBP8107-1

View Price:

OPTIZYME™ DNase I is a recombinant endonuclease that digests DNA and is RNase-Free.

Applications and Features:

OPTIZYME DNase I, RNase-Free is used for the preparation of DNA-free RNA
Removal of template DNA following in vitro transcription
DNA labeling by nick translation in conjunction with DNA Polymerase I
Preparation of DNA-free RNA prior to RT-PCR
Studies of DNA-protein interactions by DNase I, RNase-free footprinting
Enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+or Mn2+ions
Purified from non-animal host with a lower level of intrinsic RNases

Supplied with:

10X OPTIZYME DNase I Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 25mM MgCl₂, 1mM CaCl₂
50mM OPTIZYME EDTA (for inactivation by heat at 65°C for 10min)

Source: E. Coli cells with a cloned gene encoding bovine DNase I
Quality Control:

Ribonuclease-free
Functionally tested for the digestion of template DNA after in vitro transcription

Specifications

Concentration1 U/μL

ColorColorless

pH7.5

For Use With (Application)Preparation of DNA-free RNA, removal of template DNA following in-vitro transcription, DNA labeling by nick translation in conjunction with DNA Polymerase I, Preparation of DNA-free RNA prior to RT-PCR, Studies of DNA-Protein interactions by DNase I, RNase-free footprinting

Storage BufferOPTIZYME™ DNase I Buffer 10X : Tris HCl 100mM (7.5 pH at 25°C), MgCl2 25mM, CaCl2 1mM, OPTIZYME™ EDTA 50mM (for inactivation by heat at 65°C for 10min.)

SourceE. coli cells with a cloned gene encoding bovine DNase I

Quantity1000 U

Product TypeDNase I (RNase-free)

FormLiquid

Unit Size

Contents & Storage

-20°C