OPTIZYME™ EcoRV (Eco32I), Fisher BioReagents™
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OPTIZYME™ EcoRV (Eco32I), Fisher BioReagents™

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Catalog number FBRBP8012-1
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5'...G A T^A T C...3'
3'...C T A^T A G...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 50 - 100%
  • Buffer 3: 50 - 100%
  • Buffer 4: 20 - 50%
  • Buffer 5: 100%
Storage Buffer:
  • 25mM Tris-HCl (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of DNA fragments can be ligated and re-cut after a 50-fold over-digestion with EcoRV.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of EcoRV is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: May overlap - no effect
    • 5'...m5C GATATm5C G...3'
  • 3'... Gm5CTATA Gm5C...5'
  • (No effect on cleavage)
  • EcoKI: Never overlaps - no effect.
  • EcoBI: May overlap - effect not determined

    • Specifications
    Concentration10 U/μL
    Components25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
    Incubator Temperature37°C
    pH7.4
    For Use With (Application)>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRV
    Storage Buffer25mM Tris HCl (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    Quantity3000 U
    Cut SiteGAT.ATC
    Product TypeEcoRV (Eco32I)
    FormLiquid
    Unit Size
    Contents & Storage
    Keep container tightly closed