OPTIZYME™ HaeIII (BsuRI), Fisher BioReagents™

5'...G G^C C...3'
3'...C C^G G...5'

Conditions for 100% Activity:

• 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂ , 100mM KCl and 0.1mg/ml BSA.
• Incubate at 37°C.

Supplied with: 10X OPTIZYME Buffer 5
Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 0 – 20%
• Buffer 2: 0 - 20%
• Buffer 3: 0 - 20%
• Buffer 4: 50 - 100%
• Buffer 5: 100%

Storage Buffer:
10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with HaeIII.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.