Fluorescein isothiocyanate, isomer 1, 95%

Name: Fluorescein isothiocyanate, isomer 1, 95%
SKU: ALFL09319.MD

CAS: 3326-32-7 | C21H11NO5S | 389.381 g/mol

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250 mg

50 mg

Catalog number ALFL09319.MD

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Specifications

Chemical Name or MaterialFluorescein isothiocyanate

Name Noteisomer 1

CAS3326-32-7

Health Hazard 1H302-H315-H317-H319-H334-H335

Health Hazard 2GHS H Statement
H334-H317
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
May cause an allergic skin reaction.

Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

Solubility
Slightly soluble in DMSO, ethanol, methanol DMF and acetone

Notes
Incompatible with strong oxidizing agents, strong bases and amines.

RUO – Research Use Only

General References:

Reagent for fluorescent labelling of proteins: Anal. Biochem., 20, 178 (1968).
Guilini, C.; Baehr, C.; Schaeffer, E.; Gizzi, P.; Rufi, F.; Haiech, J.; Weiss, E.; Bonnet, D.; Galzi, J. New Fluorescein Precursors for Live Bacteria Detection. Anal. Chem. 2015, 87 (17), 8858-8866.
Chen, Y.; Yang, C.; Li, T.; Zhang, M.; Liu, Y.; Gauthier, M. A.; Zhao, Y.; Wu, C. The Interplay of Disulfide Bonds, α-Helicity, and Hydrophobic Interactions Leads to Ultrahigh Proteolytic Stability of Peptides. Biomacromolecules 2015, 16 (8), 2347-2355.