Kanamycin, 50 mg/ml in distilled water, sterile-filtered
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Kanamycin, 50 mg/ml in distilled water, sterile-filtered
Kanamycin, 50 mg/ml in distilled water, sterile-filtered
Kanamycin, 50 mg/ml in distilled water, sterile-filtered
Thermo Scientific Chemicals

Kanamycin, 50 mg/ml in distilled water, sterile-filtered

Kanamycin monosulfate, CAS # 25389-94-0, is an aminoglycoside sulfate salt with antibacterial activity.
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Catalog NumberQuantity
ALFJ67354.AD50 mL
Catalog number ALFJ67354.AD
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50 mL
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Chemical Identifiers
CAS125-20-2
IUPAC Name3,3-bis[4-hydroxy-2-methyl-5-(propan-2-yl)phenyl]-1,3-dihydro-2-benzofuran-1-one
Molecular FormulaC28H30O4
InChI KeyLDKDGDIWEUUXSH-UHFFFAOYSA-N
SMILESCC(C)C1=CC(=C(C)C=C1O)C1(OC(=O)C2=CC=CC=C12)C1=CC(C(C)C)=C(O)C=C1C
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SpecificationsSpecification SheetSpecification Sheet
Appearance (Form)Crystalline powder
Sulfated ash=<0.5 %
Visual transition intervalfrom pH 8.8 (colorless) to pH 10.5 (blue)
Appearance (Color)White
Infrared spectrumConforms
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It has been used in tissue culture media. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. It can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100mg/mL. Kanamycin can be used as a selection agent in several types of isolation media.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Kanamycin monosulfate is the sulfate salt form of kanamycin A
• It was isolated from Streptomyces kanamyceticus
• This compound inhibits translocation during protein biosynthesis. It binds to the 30S ribosomal subunit, causing misreading of the mRNA sequence

Application

• Kanamycin monosulfate shows antibacterial activity. It is effective against gram-positive and gram-negative bacteria, as well as Mycoplasma species
• This compound is also involved in translating prokaryotic ribosomes
• In molecular biology experiments, it is commonly used to select and isolate transformants expressing the kanamycin resistance gene, and plants that have the NPT II (APH3) gene incorporated

RUO – Research Use Only

General References:

  1. Patel, K. N.; Limgavkar, R. S.; Raval, H. G.; Patel, K. G.; Gandhi, T. R. High-Performance Liquid Chromatographic Determination of Cefalexin Monohydrate and Kanamycin Monosulfate with Precolumn Derivatization. J. Liq. Chromatogr. Related Technol. 2015, 38 (6), 716-721.
  2. Chi, Z.; Rong, Y. J.; Li, Y.; Tang, M. J.; Chi, Z. M. Biosurfactins production by Bacillus amyloliquefaciens R3 and their antibacterial activity against multi-drug resistant pathogenic E. coli. Bioprocess. Biosyst. Eng. 2015, 38 (5), 853-861.