Guanidine hydrochloride, Molecular Biology Grade

Name: Guanidine hydrochloride, Molecular Biology Grade
SKU: ALFJ65661.22

A protein denaturant

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100 g

500 g

Catalog number ALFJ65661.22

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100 g

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Specifications

Chemical Name or MaterialGuanidine hydrochloride

CAS50-01-1

Health Hazard 1Serious eye damage/eye irritation (category 2), Skin corrosion/irritation (category 2), Acute toxicity (category 4)

Health Hazard 2Causes serious eye irritation, May form combustible dust concentrations in air, Causes skin irritation, Harmful if swallowed or if inhaled

Melting Point178°C to 185°C

Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Solubility
Freely soluble in water

Notes
Protect from heat. Store away from oxidizing agents.

RUO – Research Use Only

General References:

Dipti Arora.; Navin Khanna. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology. 1996, 52, (2), 127-133.
Cing-Yuen Won.; Maurice R. Eftin. Incorporation of Tryptophan Analogues into Staphylococcal Nuclease, Its V66W Mutant, and Δ137-149 Fragment: Spectroscopic Studies. Biochemistry. 1998, 37, (25), 8938-8946.