Sodium citrate, 0.5M buffer soln., pH 6.5
Sodium citrate, 0.5M buffer soln., pH 6.5
Thermo Scientific Chemicals

Sodium citrate, 0.5M buffer soln., pH 6.5

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Catalog NumberQuantity
ALFJ63888.AK250 mL
Catalog number ALFJ63888.AK
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Quantity:
250 mL
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Chemical Identifiers
CAS480-40-0
IUPAC Name5,7-dihydroxy-2-phenyl-4H-chromen-4-one
Molecular FormulaC15H10O4
InChI KeyRTIXKCRFFJGDFG-UHFFFAOYSA-N
SMILESOC1=CC(O)=C2C(=O)C=C(OC2=C1)C1=CC=CC=C1
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SpecificationsSpecification SheetSpecification Sheet
Melting point284°C to 290°C
Infrared spectrumConforms
Loss on drying=<1.0 %
Appearance (Color)Yellow
HPLC>=99.0 %
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Sodium citrate is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0.1X to 20X depending on the application.  It is used to facilitate transfer of nucleic acids to membranes in southern and northern blotting, dot blotting, and colony and plaque lifts. 

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Sodium citrate is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0.1X to 20X depending on the application.  It is used to facilitate transfer of nucleic acids to membranes in southern and northern blotting, dot blotting, and colony and plaque lifts. 

Solubility
Soluble in water.

Notes
20X SSC Solution (RNase-free) should be stored at room temperature. This reagent should remain stable for at least 1 year in its unopened container.
RUO – Research Use Only

General References:

  1. CJ Quaife.; SD Findley.; JC Erickson.; GJ Froelick. Induction of a new metallothionein isoform (MT-IV) occurs during differentiation of stratified squamous epithelia. Biochemistry. 1994, 33,(23), 7250-7259.
  2. M Cappello.; S Keshav.; C Prince.; DP Jewell.; S Gordon. Detection of mRNAs for macrophage products in inflammatory bowel disease by in situ hybridization. Gut . 1992, 33,(9), 1214-1219.