Acrylamide/Bisacrylamide Solution (40%, 37.5:1)
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Acrylamide/Bisacrylamide Solution (40%, 37.5:1)
Thermo Scientific Chemicals

Acrylamide/Bisacrylamide Solution (40%, 37.5:1)

Acrylamide/Bisacrylamide Solution (40%, 37.5:1), also known as bioAcryl-P (40%, 37.5:1), is a premixed solution containing acrylamide and N,N'-methylenebisacrylamide (in a ratio of 37.5:1, respectively) used for preparing protein DNA gels.
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Quantity:
500 mL
1 L
Catalog number ALFJ60868.AP
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Quantity:
500 mL
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Chemical Identifiers
CAS557-34-6
IUPAC Namezinc(2+) diacetate
Molecular FormulaC4H6O4Zn
InChI KeyDJWUNCQRNNEAKC-UHFFFAOYSA-L
SMILES[Zn++].CC([O-])=O.CC([O-])=O
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SpecificationsSpecification SheetSpecification Sheet
Total Metal Impurities0.02% max.
Acrylamide/Bisacrylamide premixed solution is used for preparing protein DNA gels. It is used as a DNA sequencing gels, Protein separation. They are also used for preparing gels using stock solutions with the most common cross-link ratios.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Acrylamide/Bisacrylamide solution is used to to separate proteins
• The ratio of bisacrylamide to acrylamide modulates the porosity of the polyacrylamide gel

Application

• Acrylamide/Bisacrylamide Solution is used as a DNA sequencing gel promoting protein separation in the electrophoretic gels
• This solution is also applied for preparing gels using stock solutions with the most common cross-link ratios
• It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods

RUO – Research Use Only

General References:

  1. H Eshaghpour.; DM Crothers. Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography. Nucl. Acids Res. 1978, 5, (1), 13-21.
  2. M Bazile, HA Nichols, JA Pojman. Effect of orientation on thermoset frontal polymerization. Journal of Polymer. 2002, 40, (20), 3504-3508.